Transcriptomic profiling of blood memory B cell subsets in kidney transplant recipients

We compared the transcriptomic profile between patients without donor-specific antibodies (DSA-), patients with donor-specific antibodies without antibody-mediated rejection (DSA+ ABMR-) and patients with donor-specific antibodies and antibody-mediated rejection (DSA+ ABMR+). We found that patients with DSA+ ABMR+ had a distinct transcriptomic profile compared to DSA- and DSA+ ABMR- patients, characterized by upregulation of genes related to B cell activation and B cell effector functions. We analysed N=3 resting memory (RM, CD19+ CD38lo CD27+ CD21+), N=3 activated memory (AM, CD19+ CD38lo CD27+ CD21-) and N=3 tissue-like memory B cells (TLM, CD19+ CD38lo CD27- CD21) samples per group of patients (DSA-, DSA+ABMR-, DSA+ ABMR+), without replicates, sequencing was done using the Illumina NextSeq 500 as the platform, and RNA extraction method was Total RNA using the miRNeasy Mini kit We analysed allograft biopsy samples from N=7 DSA-, N=2 DSA+ABMR-, N=12 DSA+ ABMR+ patients, without replicates, sequencing was done using the Illumina NextSeq as the platform, and RNA extraction method was performed using RNeasy FFPE Kit Qiagen **RAW DATA NOT PROVIDED DUE TO PATIENT PRIVACY CONCERNS**