Bacterial adhesion of E. coli strains increases in influenza virus-infected cells.

Caco-2 cells were infected with influenza virus (PR8) at different MOI (0.2, 0.4 and 0.8) for 24 hrs. Cells were then infected with E. coli LF82, LF82 ΔfimH or S15 (MOI 25) for 2 hrs. As controls, cells were mock-infected and treated with neuraminidase of Clostridium perfringens (Cl NA) or not (Ctr) before bacterial infection. Cells were then lysed and the number of colony-forming units (CFU) was determined by plating (see Methods). The mean adhesion index values of the three strains are reported. Data represent the mean ± SD of three independent experiments, each performed in triplicate.**Pvs. Ctr.Bacterial adhesion of E. coli strains increases in influenza virus-infected cells.