Effect of resistant-derived extracellular vesicles on breast cancer cells acquired drug resistance process

The acquired resistance that tumors develop during the treatment is still a central cause of cancer-associated deaths. Our study investigates the transcription changes modulated by EVs from tamoxifen- and doxorubicin-resistant breast cancer cells in sensitive cells and how these changes may induce acquired drug resistance, inhibit apoptosis, and increase survivability in the sensitive cells. To better understand the influence of drug-resistant EVs on the sensitive MCF-7 and MDA-MB-231 transcriptomes, we performed RNA sequencing (RNA-Seq) analysis in these two cell lines after exposure to tamoxifen-resistant and doxorubicin-resistant EVs for 24 h. The transcriptomic profilling was performed using 3 biological replicates of RNA from sensitive MCF-7 and MDA-MB-231 cells in comparisson with RNA from tamoxifen and doxorubicin-resistant counterparts.