We find that pre-crRNAs comprising a full-length direct repeat (full-DR-crRNA) sequence with specific stem-loop G-C base substitutions exhibit increased editing efficiencies compared with the standard
Base editors, mainly including cytidine base editors(CBE) and adenine base editors(ABE), have been widely used to modify single-base in genomic DNA. The efficiency of A-to-G towards the PAM for ABE an
