Horizontal Gene Transfer of SGI-4 (from BBS 1356) or a plasmid containing oriTSGI-4_966 (from BBS 1636) to Salmonella serovars or other Enterobacteriaceae

Conjugation efficiency of Salmonella Genomic Island 4 (SGI-4) from donor Salmonella enterica serovar I 4,[5],12:i:- strain BBS 1356 or a plasmid containing oriTSGI-4_966 from donor Salmonella enterica serovar I 4,[5],12:i:- strain BBS 1636 to recipient strains of Salmonella enterica serovars (Choleraesuis, Infantis, Enteritidis, and Senftenberg) or other Enterobacteriaceae (Escherichia coli and Citrobacter youngae). The oriTSGI-4_966 nucleotide sequence spans nucleotides 4704810-4705775 of the Salmonella enterica serovar I 4,[5],12:i:- strain USDA15WA-1 genome (CP040686). Conjugation efficiency was calculated by dividing the colony forming units (CFU)/mL of transconjugants divided by the CFU/mL of donors. A transconjugant is a bacterial recipient that received DNA from the donor strain due to horizontal gene transfer.

研究内容为从供体沙门氏菌血清型I 4,[5],12:i:-菌株BBS 1356的基因组岛4（SGI-4）或含有oriTSGI-4_966的质粒（来源于供体沙门氏菌血清型I 4,[5],12:i:-菌株BBS 1636）与沙门氏菌血清型（猪霍乱沙门氏菌、婴儿沙门氏菌、肠炎沙门氏菌和森藤贝格沙门氏菌）或其他肠杆菌科细菌（大肠杆菌和克雷伯氏菌属的C. youngae）的受体菌株之间的接合效率。oriTSGI-4_966核苷酸序列覆盖了沙门氏菌血清型I 4,[5],12:i:-菌株USDA15WA-1基因组（CP040686）的4704810至4705775碱基。接合效率通过将转接合子（由供体菌株通过水平基因转移获得DNA的细菌受体）的菌落形成单位（CFU）/毫升数除以供体的CFU/毫升数来计算。