Brassica rapa Chiifu-401-42 genome sequencing project. Brassica rapa cultivar:Chiifu-401-42

We have sequenced the B. rapa accession Chiifu-401-42, an inbreed Chinese cabbage line. Genomic DNA was isolated from calli grown without light at 25deg on MS medium for 3 weeks using a standard CTAB extraction method. Diploid Brassica species B. rapa,shows great morphological plasticity. This has led to their domestication and selective breeding resulting in a range of different crop types. This morphological diversity makes B. rapa an excellent species for the study of plant morphology evolution as well as the process of domestication and directed selection. Furthermore, the genome triplication existed in B. rapa made it a good source for investigating the mechanism of whole genome triplication in plants. Thus we believe this genome to be of scientific interest to the broader communities of comparative genomics and eukaryotic evolution, in addition to those interested in crop breeding, food security, health and industrial products.