Fatty acid profiling of Pseudorhizobium strains

Fatty acid profilesFatty acid methyl esters were obtained as previously described (Kämpfer & Kroppenstedt 1996) and separated by using a gas chromatograph (model 6890 N; Agilent Technologies). Peaks were automatically computed and assigned using the Microbial Identification software package (MIDI), TSBA40 method, Sherlock version 6.1. The dendrogram was created with Sherlock Version 6.1. Polar lipids and respiratory lipoquinonesPolar lipids and respiratory lipoquinones were extracted from 100 mg freeze-dried cells and separated by two dimensional silica gel thin layer chromatography by the identification service of the DSMZ as previously described (Hahnke et al. 2012).