DataSheet_1_Tissue Trafficking Kinetics of Rhesus Macaque Natural Killer Cells Measured by Serial Intravascular Staining.pdf

The in vivo tissue distribution and trafficking patterns of natural killer (NK) cells remain understudied. Animal models can help bridge the gap, and rhesus macaque (RM) primates faithfully recapitulate key elements of human NK cell biology. Here, we profiled the tissue distribution and localization patterns of three NK cell subsets across various RM tissues. We utilized serial intravascular staining (SIVS) to investigate the tissue trafficking kinetics at steady state and during recovery from CD16 depletion. We found that at steady state, CD16+ NK cells were selectively retained in the vasculature while CD56+ NK cells had a shorter residence time in peripheral blood. We also found that different subsets of NK cells had distinct trafficking kinetics to and from the lymph node as well as other lymphoid and non-lymphoid tissues. Lastly, we found that following administration of CD16-depleting antibody, CD16+ NK cells and their putative precursors retained a high proportion of continuously circulating cells, suggesting that regeneration of the CD16 NK compartment may take place in peripheral blood or the perivascular compartments of tissues.

自然杀伤细胞（NK细胞）在体内组织分布及其迁移模式尚处于研究不足的状态。动物模型有助于填补这一研究空白，而恒河猴（RM）灵长类动物能够忠实地重现人类NK细胞生物学中的关键要素。本研究中，我们对三种NK细胞亚群在多种恒河猴组织中的组织分布和定位模式进行了分析。我们采用了连续血管内染色（SIVS）技术，以探究在稳态和CD16耗竭恢复期间的组织迁移动力学。研究发现，在稳态下，CD16+ NK细胞被选择性地保留在血管中，而CD56+ NK细胞在周围血液中的停留时间较短。此外，我们还发现不同的NK细胞亚群在淋巴节点及其他淋巴和非淋巴组织之间的迁移动力学存在差异。最后，我们发现，在给予CD16耗竭抗体后，CD16+ NK细胞及其潜在的前体细胞保留了较高比例的持续循环细胞，这表明CD16 NK细胞库的再生可能发生在外周血液或组织血管周围腔隙中。