RNA sequencing Facilitates Quantitative Analysis of ANGPTL8 on Lipid metabolism in liver of chow mice. RNA sequencing Facilitates Quantitative Analysis of ANGPTL8 on Lipid metabolism in liver of chow mice

We report the use of transcriptome sequencing in the intervention of ANGPTL8 in the regulation of triglyceride level. We constructed the adenovirus-mediated overexpression and knockdown of ANGPTL8 in wild-type mice and obtained the differential genes of ANGPTL8 regulating serum triglyceride level by transcriptomic sequencing. We identified 234 differentially expressed ANGPTL8 response genes, 113 genes are up-regulated in ANGPTL8 overexpression，and down-regulated in ANGPTL8 knock-down. 121 genes are up-regulated in ANGPTL8 down-knock, and down-regulated in ANGPTL8 overexpression. This study provides a framework for the network regulation mechanism of ANGPTL8 controlling triglyceride level. Overall design: Exploring the Signal Network of ANGPTL8 Regulating Lipid Metabolism Through RNA sequencing