Quantitative Proteomics Analysis Confirmed Oxidative Metabolism Predominates in Streptomyces coelicolor versus Glycolytic Metabolism in Streptomyces lividans

Recent physiological studies indicated
that S. lividans metabolism was mainly glycolytic,
whereas S. coelicolor metabolism was mainly oxidative.
To determine whether such metabolic
characteristics were correlated with consistent proteomics features,
a comparative label-free, shotgun proteomics analysis of these strains
was carried out. Among 2024 proteins identified, 360 showed significant
differences in abundance between the strains. This study revealed
that S. coelicolor catabolized glucose less actively
than S. lividans, whereas the amino acids present
in the medium were catabolized less actively by S. lividans than by S. coelicolor. The abundance of glycolytic
proteins in S. lividans was consistent with its high
glycolytic activity, whereas the abundance of proteins involved in
the catabolism of amino acids in S. coelicolor provided
an explanatory basis for its predominantly oxidative metabolism. In
this study, conducted under conditions of low O2 availability,
proteins involved in resistance to oxidative stress and those belonging
to a DosR-like dormancy regulon were abundant in S. coelicolor, whereas tellurium resistance proteins were abundant in S. lividans. This indicated that the strains reacted differently
to O2 limitation. Proteins belonging to the CDA, RED, and
ACT pathways, usually highly expressed in S. coelicolor, were not detected under these conditions, whereas proteins of siderophores,
5-hydroxyectoine, and terpenoid biosynthetic pathways were present.

近期生理学研究表明，S. lividans 的代谢以糖酵解为主，而 S. coelicolor 的代谢则以氧化代谢为主。为探究此类代谢特性是否与一致的蛋白质组学特征相关，本研究对这两种菌株进行了无标记的质谱蛋白质组学分析。在鉴定的2024种蛋白质中，有360种在菌株间表现出显著的丰度差异。研究揭示，S. coelicolor 对葡萄糖的分解作用不如 S. lividans 活跃，而培养基中存在的氨基酸在 S. lividans 中的分解作用不如在 S. coelicolor 中活跃。S. lividans 中糖酵解蛋白质的丰度与其高糖酵解活性相一致，而参与氨基酸分解的蛋白质在 S. coelicolor 中的丰度为其以氧化代谢为主的代谢特性提供了解释依据。在低氧环境下进行的本研究中，参与抗氧化应激的蛋白质以及属于 DosR-like 休眠调控子的蛋白质在 S. coelicolor 中含量丰富，而抗碲蛋白质在 S. lividans 中含量丰富。这表明两种菌株对氧限制的反应不同。通常在 S. coelicolor 中高表达的 CDA、RED 和 ACT 途径的蛋白质在此条件下未检测到，而铁载体、5-羟基乙内酯和萜类生物合成途径的蛋白质则存在。