the Role of Sugar-Sweetened Beverages in the Progression of a Murine Metabolic Dysfunction-Associated Steatotic Liver Disease Model

This study establishes a murine model to dissect SSB-specific contributions to MASLD progression. Eight-week-old male C57BL/6N mice were fed a high-fat high-cholesterol (HFHC) diet with/without fructose-, glucose-, or sucrose-sweetened beverages for 10 weeks. Hepatic transcriptomic profiles were analyzed via microarray, followed by functional enrichment. protein-protein interaction (PPI) network and single-cell analysis identify pathway perturbations and hub genes.