Evaluation of various enzymatic assays for glucose dehydrogenase activity of GDH1E5.

a All data represent the average of triplicate determinations ± standard derivation.All enzyme assays were carried out using purified GDH1E5 over 5 min at 35°C and 75 mM glucose as a substrate. NAD(P)+ was used in concentrations of 5 mM respectively. The formation of reduced NAD(P)H was followed by an increase in absorbance at 340 nm. The enzymatic reduction of p-nitrosoaniline BM53.0861 and DCPIP was followed by an increase in absorbance at 620 nm for the nitrosoaniline and a decrease in absorbance at 600 nm for the dichloroindophenol.