Analysis of Brain Protein Stability Changes in Mouse Models of Alzheimer’s Disease

. This study employed different mass spectrometry-based proteomic methods to characterize Alzheimer's Disease (AD) research by examining protein folding and stability in mouse models, offering a fresh perspective beyond traditional protein expression level analysis. Stability of Proteins from Rates of Oxidation (SPROX), Thermal Proteome Profiling (TPP), and Limited Proteolysis (LiP) are used to profile protein stability and conformational change in the hippocampus tissue cell lysates between wild type (n=7) and 5XFAD transgenic (n=7) mice at two time points (t1=2-month, t2=8-month).