Efficiency of cloning of pX-GFP at different restriction-ligation times.
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Restriction/ligations were performed from 5 to 30 minutes, and either immediately transformed in E.coli (Digest an heat inactivation/no) or incubated 5 minutes at 50°C and 5 minutes at 80°C (Digest an heat inactivation/yes) before transformation in E.coli.*The zero minute time point was performed without adding BsaI to the restriction-ligation mix.
创建时间：
2015-12-02
