Identification of intragenic suppressors.
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A plasmid encoding CpxAΔP, a constitutive kinase CpxA variant lacking the sensor domain, was mutagenized and transformed into a cpxA null PcpxP–lacZ recA strain background. The table shows the CpxA activity conferred by single mutant cpxA alleles in the NS54 strain. Point mutations were identified from colonies displaying a Lac− phenotype in X-gal containing plates.
创建时间：
2015-12-02
