Experimental evaluation of farnesylation of predicted peptide substrates: 26/29 (90%) of the predictions are indeed farnesylated, including a novel class of farnesylation targets identified in this study.

(A) Top-scoring peptides. (B) Top-scoring peptides that occur at C-termini of human proteins.
The novel class of farnesylation targets identified in this study that contains acidic C-terminal residues (see Figure 4D) are shown in bold.
aPeptide score for sequences as measured by the FlexPepBind protocol developed in this study.
bExperimental validation of farnesylation of predicted peptides in this study (see Methods).
cUniprot [24] identifier of human proteins containing putative farnesylation motif.
d-fPrePS predictions [7]:
dbased on 30 C-terminal residues of protein sequence;
ebased on 30 C-terminal residues of protein, with the last 4 residues replaced by the H-Ras canonical Cxxx motif (CVLS) (this indicates the amenability of the upstream sequence to allow farnesylation of the C-terminus);
fbased on 30 C-terminal residues of known substrate H-Ras, with last 4 residues replaced by Cxxx motif (this indicates the amenability of the given Cxxx C-terminal sequence to undergo farnesylation within the context of a known strong farnesylation target).