Heading towards a dead end: the role of DND1 in germ line differentiation of human iPSCs

We knocked out DND1 in human iPSCs and analysed its role in pluripotency and differentiation. We showed that general pluripotency gene expression is not affected, but KO iPSCs differentiate less efficiently towards PGC-like cells and remaining PGCLCs show reduced expression of NANOS3 and PRDM1. Overall design: We examine the effects of DND1 KO during human germ cell differentiation. Experiments were performed in iPSC, iMeLCs and PGCLC. For each cell type there is one Wild Type (WT) sample and three DND1 Knock Outs (KO). Each set of samples is run in duplicates.