Figure_4_replicates
收藏NIAID Data Ecosystem2026-05-01 收录
下载链接：
https://figshare.com/articles/dataset/Figure_4_replicates/22705423
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资源简介：
(B, F, G) HEK293T ishXrn1cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing insertions of the indicated lengths from the YKT6 or STOML2 genes (B). In F and G, constructs also carry mutations that disrupt the hairpin structure and restore with a different sequence. PR8 PA-X or an empty vector were also co-transfected. RNA was extracted to run 5’ RACE. DNA bands were purified and sequenced to confirm their identities.
R# --> replicate number; AI#/LG### --> experiment number.
.sgd are the original source files acquired with the Syngene Imager.
创建时间：
2023-05-01
