Sequences of primers used in qRT-PCR
收藏Figshare2024-10-23 更新2026-04-08 收录
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https://figshare.com/articles/dataset/Sequences_of_primers_used_in_qRT-PCR/27283434/1
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资源简介：
To measure mRNA levels of inflammatory cytokines tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6), total RNA was extracted from substantia nigra tissues using the RNeasy Mini Kit (QIAGEN). cDNA synthesis was performed with Superscript III Reverse Transcriptase (Fermentas), and quantitative real-time PCR was used to detect cytokine mRNA levels. β-actin served as an internal control for normalization. Gene expression was calculated with the 2−ΔΔCT method. Primer details are in <b>Table 1</b>.
提供机构：
Liu, Xiaomin
创建时间：
2024-10-23
