Effect of hypoxia (1% oxygen for 24 hours) on the expression and transcription of human HeLa, U87, MCF7 and HCT-116 cells

Mitochondria respond to various stresses. Nevertheless, the regulation of this response while considering coordination between mitochondrial (mtDNA)- and nuclear DNA (nDNA)-encoded gene expression has been overlooked. To analyze the mito-nuclear gene expression response to hypoxia, RNA-seq was performed on HeLa, U87, MCF7 and HCT-116 cells exposed to 24 hours of 1% hypoxia and matching controls grown for 24 hours under 21% oxygen. Additionally, nascent RNA transcripts analysis (PRO-seq) in HeLa, U87 and D407 cells was performed under similar conditions.