Expression data from CRC patient-derived organoids

Colorectal cancer (CRC) is a commonly occurring cancer worldwide. Metastasis and recurrence are the major causes of cancer-related death. CRC progression is a multistep process, and extensive efforts have been made to identify the genomic and transcriptomic alterations that occur during this process. However, whether primary tumors and metastatic lesions possess distinct biological features remains unclear. We established 74 patient-derived organoids (PDOs) from primary tumors and patient-matched metastatic and recurrent lesions. CRC PDOs were isolated for RNA extraction and hybridization on Affymetrix microarrays. In addtion to 74 PDOs, two organoids derived from nomal mucosa were included.