Transcriptomic analysis of EBV-negative Akata Burkitt Lymphoma Cell target genes upon CRISPR knockout of the individual NF-?B transcription factor as well as induction of wildtype LMP1 expression

RNA-seq was used to characterize the NF-?B transcription factor -mediated regulation of B-cell genome wide target genes upon inducible LMP1 expression . We created an inducible stable EBV-negative Akata Burkitt Lymphoma cell line expressing LMP1 wildtype followed by stable CRISPR knockout of the individual NF-?B transciption factors (p52, RelB, p50, cRel or RelA) and 24 hours 250ng/ml doxycycline-induced LMP1 expression. Overall design: Comparative gene expression profiling analysis of RNA-seq data EBV-negative Akata Burkitt Lymphoma Cells upon stable CRISPR knock out each of the five NF-?B transcription factor (p50,p52, RelB, cRel or RelA) and inducible expression of the wildtype LMP1. Stable cell line with wildtype LMP1 induced expression and bearing a non-targeting control is used as the control