Data for: Comparative proteomics of geographically distinct saw-scaled viper (Echis carinatus) venoms from India

We investigated on venom variation in geographically distinct populations of Echis carinatus from three ecologically distinct regions: Tamil Nadu (ECVTN), Goa (ECVGO), and Rajasthan (ECVRAJ). Venom was fractionated by RP-HPLC, combined with SDS-PAGE, and subjected to tandem mass spectrometry. Toxins were identified, and their relative abundance was estimated. Using NCBI database of Echis genus, we queried the MS/MS spectra, and found 87, 44 and 48 proteins, in ECVTN, ECVGO and ECVRAJ respectively, belonging to 7-9 different toxin families. The differences in the venom profiles were due to change in the relative composition of the toxin families.

本研究针对来自三个生态迥异地区——泰米尔纳德邦（ECVTN）、果阿（ECVGO）和拉贾斯坦邦（ECVRAJ）的土鳖蜥（Echis carinatus）地理种群中的毒液变异进行了探究。毒液通过反相高效液相色谱法（RP-HPLC）进行分级，结合聚丙烯酰胺凝胶电泳（SDS-PAGE）分析，并采用串联质谱法进行检测。通过鉴定毒素并估算其相对丰度。利用NCBI（美国国家生物技术信息中心）的土鳖蜥属数据库，我们对质谱/质谱（MS/MS）谱图进行查询，在ECVTN、ECVGO和ECVRAJ中分别发现了87、44和48种蛋白质，它们分别属于7-9个不同的毒素家族。毒液谱图差异归因于毒素家族相对组成的改变。