NKAPL binding sites in mouse testis and the genome-wide occupancy of the SOX30/HDAC3 protein complex and RNA Pol II in the absence of NKAPL

To profile genomic binding sites of NKAPL and examine whether its knockout alters the genome-wide occupancy of the SOX30/HDAC3 protein complex and RNA Pol II in mouse testes, wild-type and Nkapl KO testes at postnatal day 21 were harvested for ChIP-seq. Overall design: Wild-type and Nkapl KO mouse testes were havested at postnatal day 21. ChIP experiments were performed with the indicated antibodies for NKAPL, SOX30, HDAC3, and RNA Pol II. Precipitated DNA was amplified according to ChIP Sequencing Sample Preparation Guide (Illumina).