Proteomics coupled with in vitro model to study the early crosstalk occurring between newly excysted juveniles of Fasciola hepatica and host intestinal cells

Here, we develop a new in vitro model replicating the first interaction of FhNEJ with mouse primary small intestinal epithelial cells (MPSIEC). FhNEJ and MPSIEC were incubated for 3 hours and protein extracts (tegument and soma for FhNEJ and membrane and cytosol for MPSIEC) were subjected to quantitative SWATH-MS proteomics compared with respective controls to evaluate the changes in both the parasite and the host.