scMMO-atlas

Here, we constructed a single-cell multimodal omics (scMMO) atlas by collecting and integrating various scMMO data, then constructed scMMO database and portal called scMMO-atlas (https://www.biosino.org/scMMO-atlas/). scMMO-atlas includes scATAC+RNA (ISSAAS-seq, SNARE-seq, paired-seq, sci-CAR, scCARE-seq, 10X Multiome and so on), scRNA+protein, scATAC+protein, and scTri-modal omics data, with 3,168,824 cells from 27 cell tissues/organs. scMMO-atlas offered an interactive portal for visualization and featured analysis for each modality and the integrated data. Integrated analysis of scATAC+RNA data of mouse cerebral cortex in scMMO-atlas identified more cell subsets compared with unimodal omics data. Among these new cell subsets, there is an early astrocyte subset highly expressed Grm3, called Astro-Grm3. Furthermore, we identified Ex-L6-Tle4-Nrf1, a progenitor of Ex-L6-Tle4, indicating the statistical power provided by the big data in scMMO-atlas. In summary, scMMO-atlas offers cell atlas, database and portal to facilitate data utilization and biological insight.本研究通过收集并整合多种单细胞多组学（single-cell multimodal omics，简称scMMO）数据，构建了单细胞多组学图谱，并据此开发了名为scMMO-atlas的数据库与交互门户（https://www.biosino.org/scMMO-atlas/）。scMMO-atlas涵盖了scATAC+RNA（包含ISSAAS-seq、SNARE-seq、paired-seq、sci-CAR、scCARE-seq、10X Multiome等技术）、scRNA+蛋白、scATAC+蛋白以及三模态单细胞组学数据，共包含来自27种细胞组织/器官的3168824个细胞。scMMO-atlas提供了交互式门户，支持对各模态数据及整合后的数据进行可视化与特色分析。通过对scMMO-atlas中小鼠大脑皮层的scATAC+RNA数据进行整合分析，相较于单模态组学数据，本研究鉴定出了更多的细胞亚群。在这些新鉴定的细胞亚群中，存在一个高表达Grm3的早期星形胶质细胞亚群，命名为Astro-Grm3。此外，本研究还鉴定出了Ex-L6-Tle4的祖细胞亚群Ex-L6-Tle4-Nrf1，这体现了scMMO-atlas的大数据所具备的统计效力。综上，scMMO-atlas提供了细胞图谱、数据库与交互门户，以助力数据利用与生物学发现。