ExtFig4A_RACE_D108A_PA_R2-R3_LG384-387.tif
收藏Figshare2023-01-19 更新2026-04-08 收录
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https://figshare.com/articles/dataset/ExtFig4A_RACE_D108A_PA_R2-R3_LG384-387_tif/21810189/1
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资源简介：
HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with the luciferase reporters containing 99 bp insertions from the STOML2, and where indicated, with PR8 PA-X, PR8 PA or a PR8 PA expressing construct with a mutation in the frameshift sequence to prevent PA-X expression (PA(fs)). RNA was extracted and used to run 5’ RACE with primers annealing to luciferase sequences. DNA bands were purified and sequenced to confirm their identities
提供机构：
Gaglia, Marta
创建时间：
2023-01-19
