Heterotrophic nanoflagellate and bacteria abundance in water samples collected at Station MARCH-1997-GN36199704601MSB01 in the Aegean Sea

Subsamples (10-30 ml) were concentrated on 25 mm black polycarbonate filters (pore size 0.2 µm), stained with DAPI for 10 min (Porter, Feig, 1980) and filtered. Under epifluorescence microscopy heterotrophic bacteria were counted using UV excitation.