Inhibition of a G9a/DNMT network triggers an immune-mediated bladder cancer regression

Using a novel immunocompetent quadruple (PtenF/F; Trp53F/F; Rb1F/F;Rbl1-/-) knockout transgenic mouse model of highly aggressive metastatic muscle-invasive BC, we demonstrate that treatment with CM272 and CDDP results in a significant tumor and metastasis regression with a very significant prolonged survival Gene expression was compared between normal bladders from transgenic CKO mice (Rbf/f, Ptenf/f, Tp53f/f, p107-/- ), tumor bladders from CKO inoculated with Adeno-Cre in the bladder, bladders from CKO treated with CM272+CDDP, and metastasis from these mice.