Supplementary materials for Barrett et al., 2021

Supplementary material describing comparisons between two RNA-sequencing library preparation techniques for low input samples in <i>C. elegans</i>. Two techniques were compared head-to-head on the same samples, a PolyA selection based approach (SMARTseq V4, Takara), and a ribodepletion based approach (SoLo Ovation, Tecan Genomics) with a custom ribodepletion probe set designed for <i>C. elegans</i>.<br>Here we describe: correlation analyses within and between each kit; gene level variance measures compared between the two techniques for all protein coding genes; and gene level variance comparisons between both techniques for the 2000 shortest and longest protein coding genes.<br>We also provide supplementary tables describing: metrics for sample inputs and mapping rates for all samples; mean and median values of variance measures in 5 ranked groups for both techniques, and comparisons between sample preparation in this study and a previous study.