The Human Protein Atlas

The Swedish Human Protein Atlas project has been set up to allow for a systematic exploration of the human proteome using Antibody-Based Proteomics. This is accomplished by combining high-throughput generation of affinity-purified antibodies with protein profiling in a multitude of tissues and cells assembled in tissue microarrays. Confocal microscopy analysis using human cell lines is performed for more detailed protein localization. The program hosts the Human Protein Atlas portal with expression profiles of human proteins in tissues and cells. The main objective of the resource centre is to produce specific antibodies to human target proteins using a high-throughput production method involving the cloning and protein expression of Protein Epitope Signature Tags (PrESTs). After purification, the antibodies are used to study expression profiles in cells and tissues and for functional analysis of the corresponding proteins in a wide range of platforms.

瑞典人类蛋白质图谱项目旨在通过抗体基蛋白质组学的方法，系统性地探索人类蛋白质组。该项目通过结合高通量亲和纯化抗体的生成与多种组织和细胞构成的组织微阵列中的蛋白质分析，实现了这一目标。利用人源细胞系进行的共聚焦显微镜分析，用于更详细的蛋白质定位研究。项目还托管了人类蛋白质图谱门户，该门户收录了人类蛋白质在组织和细胞中的表达谱。资源中心的主要目标是利用高通量生产方法，通过蛋白质表位特征标记（PrESTs）的克隆和蛋白质表达，生产针对人类靶蛋白的特异性抗体。经纯化后，这些抗体被用于研究细胞和组织中的表达谱，以及在广泛平台上对相应蛋白质的功能分析。

搜集汇总

背景与挑战

背景概述

The Human Protein Atlas是一个系统研究人类蛋白质组的资源库，通过抗体蛋白质组学方法生成蛋白质表达谱数据，包含超过27,800种抗体靶向17,300多个人类基因的蛋白质，为细胞生物学和医学研究提供重要资源。