DNA Synthesis in a Closed Loop Control System

A closed control loop system ensures precise acquisition of A, T, C, or G volumes for DNA synthesis. Users input a definite volumes through a software, which directs a microcontroller. This controls high-accuracy syringe pumps to draw specified base volumes. A volume sensor verifies the actual amount drawn, providing feedback for adjustment. If discrepancies occur, an error signal prompts the controller to recalibrate the actuators. This system ensures reliability and can extend to temperature and pH control using appropriate sensors. Time to achieve steady output can be optimized using a PID controller, enhancing response times. Read more ...Garg, R. , Vyas, A. , Khan, A. , Tariq, M. (2024), 'Codes beyond Bits and Bytes: A Blueprint for Artificial Life', World Academy of Science, Engineering and Technology, Open Science Index 213, International Journal of Biotechnology and Bioengineering, 18(9), 114 - 126.