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Primers used for the amplification of heavy, light Kappa (κ) and light Lambda (λ) chains.

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Figshare2025-07-01 更新2026-04-28 收录
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Three PCR reactions are performed per chain. PCR1 and PCR2 are performed with forward and reverse primer mixes. PCR3 is performed with specific primer pairs selected from 69 individual primers. The selection is based on sequencing results of the second PCR amplification and identification of used V(D)J alleles. See the attached Table S1.xlsx file. Refer also to Fig 2. (XLSX)
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2025-07-01
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