Allele-specific genome editing using the CRISPR/Cas9 system phenocopies alopecia areata symptomatic patched hair loss in mice. Mus musculus

Using CRISPR/Cas9 for allele-specific genome editing we phenocopied AA symptomatic patched hair loss in mice engineered to carry the Cchcr1 risk allele. Overall design: Fluorescent cRNA synthesis derived from eight skin RNAs was performed using Agilent’s Low RNA Input Linear Amplification kit (Agilent Technology) and subjected to DNA microarray analysis using single-color microarray-based gene-expression analysis (SurePrint G3 Mouse GE Ver 2.0, 8 x 60K, Agilent Technology). All procedures were performed according to the manufacturer’s instructions.