OMAP-7 Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Lung with Cell DIVE, v1.2

OMAP-7 was designed for Cell DIVE imaging of Formalin-Fixed Paraffin-Embedded (FFPE) lung tissue. Cell DIVE enables<br>highly multiplexed single cell analysis using antibody-based imaging (Gerdes, MJ. et al, 2013). The tissue slides were<br>prepared with a two-step antigen retrieval process (pH 6 and pH 9, as described<br>https://dx.doi.org/10.17504/protocols.io.bpwumpew. Antigens were detected using either primary antibodies with<br>fluorophore-labeled secondaries or fluorescent dye conjugated antibodies (protocol can be found<br>https://dx.doi.org/10.17504/protocols.io.b4kgqutw. The panel contains 30 antibodies, 4 of which are used for cell<br>segmentation (Na/K ATPase, Cytokeratin, Cadherin, and S6) and the nuclear marker DAPI for image alignment and nuclear<br>segmentation. This OMAP provides a spatial context for key cell types in the[ASCT+B lung table<br>v1.1](https://doi.org/10.48539/HBM323.SGDF.945). Additionally, the inclusion of protein biomarkers such as AGTR1,<br>Cathepsin L, TMPRSS2, ICAM-1, and CD55 allow characterization of proteins relevant to SARS-CoV-2 infection and<br>profiling of the renin-angiotensin system (RAS) that may play a role in COVID-19 disease. The core and essential<br>protein biomarkers detailed here overlap (~25%) with the panel developed for multiplexed imaging of lung samples<br>established using conventional automated IHC staining for FFPE-material (Dr. Christopher Werlein, an expert lung<br>pathologist). This OMAP does not contain antibodies for typing subsets of fibroblasts, macrophages, or endothelial<br>cells. A representative dataset using this OMAP can be found here: https://zenodo.org/record/7731162#.ZBmz2nbMIuU.