DNA sequences for: Synthetic control of actin polymerization and symmetry breaking in active protocells

Non-linear biomolecular interactions on membranes drive membrane remodeling crucial for biological processes including chemotaxis, cytokinesis, and endocytosis. The complexity of biomolecular interactions, their redundancy, and the importance of spatiotemporal context in membrane organization impede understanding of the physical principles governing membrane mechanics. Developing a minimal in vitro system that mimics molecular signaling and mem- brane remodeling while maintaining physiological fidelity poses a significant challenge. Inspired by chemotaxis, we reconstructed chemically regulated actin polymerization inside vesicles, guiding membrane self-organization. An external, undirected chemical input induced directed actin polymerization and membrane deformation uncorrelated with upstream biochemical cues, suggesting symmetry breaking. A biophysical model incorporating actin dynamics and membrane mechanics proposes that uneven actin distributions cause non-linear membrane deformatio..., Sequencing Files: The sequences provided were generated using standard cloning methods and were verified via Sanger sequencing for accuracy and completeness., , # DNA sequence files

Supplementary DNA Sequences for 'Synthetic Control of Actin Polymerization and Symmetry Breaking in Active Protocells'

Manuscript Title: Synthetic Control of Actin Polymerization and Symmetry Breaking in Active Protocells

Authors: Shiva Razavi, Felix Wong, Bedri Abubaker-Sharif, Hideaki T. Matsubayashi, Hideki Nakamura, Nhung Thi Hong Nguyen, Douglas N. Robinson, Baoyu Chen, Pablo A. Iglesias, and Takanari Inoue.

Access this dataset on Dryad: [https://doi.org/10.5061/dryad.rxwdbrvh9](https://doi.org/10.5061/dryad.rxwdbrvh9)

## Description of the data and file structure

This dataset comprises the DNA sequences corresponding to the proteins that were purified and utilized in this study.

The files are provided in Genebank (.gbk) format and include the following sequences:

\- 2xStrep-mActA(1-184)-FRB-CFP: Contains the sequence of ActA(1-184) codon-optimized for mammalian expression and tagged with a Strep tag for purification. The FRB module enables the transloc...