Identification of Sec23ip, part of 14-3-3γ protein network, as a regulator of acute steroidogenesis in MA-10 Leydig cells

Supplemental Figure 1.
Image obtained from protein atlas databases. Sections of a testes obtained from
a patient were stained with Sec23ip antibody. Patient data and cell types with
Sec23ip expression are indicated.

Supplemental Table 1.
A list of proteins from the 14-3-3γ interactome in cAMP-treated MA-10 cells at times
0, 30, 60 and 120. The proteins are listed based on progressive increase in
interactions with 14-3-3γ.

Supplemental Table 2.
A selected list of 14-3-3γ interactome containing proteins that showed
consistent interactions at all time points across n= 3.

Supplemental Table 3.
Relative gene expression for Sec23ip in steroidogenic tissues in human and
mice. This data was obtained from biogps database using the probeset 209175-at
for human and probeset 1433627-at for mice.

Supplemental Table 4.
The sequences of 3 siRNA used for Sec23ip knock down.