General health, neurological reflexes, and pain sensitivity.

Normal general health and pain responses in Cohort 1 of En2 mice. No genotype differences were detected using a standard battery of parameters. Data shown are means ± standard error of the mean (SEM) for body weight, temperature, wire hang latency, hot plate and tail flick latency, and behaviors assessed using a 3 point ranking scale. Percentage of mice that exhibited a specific neurological reflex or physical abnormality is expressed as percent of total mice within each genotype. N = 17+/+, N = 16+/−, N = 15−/−. Similar results were obtained for Cohort 2 (data not shown).