Doublets

To compare the use of light scattering with fluorescence measurement techniques for successful doublet discrimination for single cells by using peripheral blood samples were prepared with and without fixative and non-fixative erythrocyte lysing solutions.
Conclusion:
- The obtention of single cell preparations is crucial to characterize cell suspensions, even obtained after tissue and tumor disaggregation for flow cytometric analysis, especially when is extremely needed to detect rare events, by maximizing the signal to noise ratio and by increasing the number of acquired events. - Overall, fluorescent methods for thresholding and doublet discrimination can be very helpful to identify coincident events and to increase the resolution of singlets. - Doublet discrimination by fluorescence takes advantage not only for NLNW methods, because also simplifies the identification of aggregates, by avoiding artifacts during sample manipulation, as well as the use of inaccurate light scatter triggering for small pathological cells or early apoptotic events as a consequence of cell shrinkage. Performance Tracking Beads (daily)