Methylation of gDNA from twins with tuberculosis infection or not

To search the methylation of genes which were associated with tuberculosis infecion We used the microarrays to detail the methylation of gDNA between with the tuberculosis infection and without the tuberculosis in monozygous twins Total blood DNA was extracted using the EDTA DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions. The amount of DNA was quantified by the spectrophotometric A260 to A280 ratio of 1.8. DNA methylation patterns were analyzed using the Illumina Infinium HumanMethylation850 Bead Chip (Illumina, Inc.), according to the manufacturer’s instructions.