Small RNA sequencing in wild chickpea

We report small RNA data from the leaves of wild chickpea PI 489777. Small RNA library was prepared and sequencing was performed using Illumina platform. A total of 23 million reads were generated, which represented 0.95 million unique reads. These were mapped to the chickpea genome using Bowtie to obtain the non-redundant set of unique small RNA sequences. Overall design: In this study, RNA was isolated from the leaves of PI 489777 and small RNA library was prepared using TruSeq Small RNA Sample Prep Kit (Illumina Technologies) according to the manufacturer''s instructions. Data obtained in FASTQ files was pre-processed to remove low-quality reads. Unique reads from each sample were mapped to the chickpea genome using Bowtie.Only the non-redundant set of uniquely mapped small RNA reads were retained.