Distinct Functional Properties of Perinatal vs Adult Adipose Progenitors

We previously identified functional distinct sub-popultions in adult male gWAT Pdgfrb+ cells utilizing the MuralChaser lineage tracking system and Single-cell RNA-seq. To examine the cellular atlas and developmental aspects of male gWAT, we performed Single-cell RNA-seqs on different developing stage of gWAT (P3,P7 and 5-week). To examine the mesothelial origin hypothesis of gWAT, we also performed Single-cell RNA-seq on P7 gWAT associated mesothelial cells. We isolated Stromal Vascular Fraction (SVF) from epidydimal white adipose tissue (eWAT) of Postnatal Day 3 (n=10)/ Postnatal Day 7 (n=5) / 5-week (n=5) male mice respectively. We also isolated Postnatal Day 7 eWAT associated mesothelial cells. Single cell library preparation was performed using the 10X Genomics Single Cell 3 v3 according to the manufacturers instructions.