BRAF and MEK inhibitor therapy eliminates nestin expressing melanoma cells in human tumors Experiment 3 (Cell Lines)

To provide single-cell mass cytometry data of melanoma cell lines This panel dissects melanoma cellular heterogeneity with melanoma panel 3.0.
Conclusion:
Identified diversity among melanoma cell lines. Including the presence of the abundant nestin positive phenotype.
Notes:
These events are melanoma cell lines pre-gated as intact cells with histone H3 and rhodium intercalator as a viability agent. This analysis pairs with Figure 4 in the manuscript titled "BRAF and MEK inhibitor therapy eliminates nestin expressing melanoma cells in human tumors". These events are intact cells pre-gated with histone H3 and rhodium intercalator as a viability agent. Fluidigm bead-based normalization was performed before analysis and beads were removed before viSNE analysis.