Single cell RNA sequencing analysis of the center, edge and non-wounded regions of healing skin on day 11 post-wounding in wild type mice

We applied single-cell RNA sequencing to investigate specific type of cell populations and their gene expressions on the day of wound closure (post-wounding day 11-12) at the center and edge of the re-epithelialized mouse wound site (1 cm² of whole skin was removed, with samples pooled from 10 mice). Skin samples from non-wounded area were collected as control. Viable cells from the single-cell suspension were selected by sorting, and a single-cell library was prepared using 10x Genomics and sequenced with the Illumina NovaSeq 6000. Downstream bioinformatic analysis of single-cell RNA sequencing data was performed using the Seurat package in R. Cell clusters were annotated according to their identity using specific marker genes.