Endothelial cells mediated by STING signaling regulate oligodendrogenesis and myelination during brain development. Endothelial cells mediated by STING signaling regulate oligodendrogenesis and myelination during brain development

To gain futher insight into how endothelial STING regulates oligodendrogenesis ,RNA-seq was used to analyze the genome-wide changes resulting from isolated endothelial cells of E18 endothelial STING conditional knock out mice and littermate wild-type.mRNA from E18 isolated endothelial cells of wild-type(WT) and STINGf/f;Tie2-cre mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, mRNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 2500 platform in Annoroad Genomics Overall design: mRNA profiles of E18 wild type (WT) and STINGECKO mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.