RNA Sequencing Analysis of Wild Type and Non-phosphorylatable CARM1 mutation knock-in HEL cells

CARM1 is essential for the development and maintenance of myeloid neoplasms. We found that non-phosphorylatable CARM1 mutation impairs cell cycle progression, induces apoptosis, and downregulates stemness in JAK2-mutant cell lines, suggesting that CARM1 phosphorylation is required for maximal proliferation of myeloid neoplasms. Overall design: Gene expression profile of wild-type or non-phosphorylatable CARM1 mutation (Y149F or Y334F single, or Y149F/Y334F double mutation) knock-in HEL cells.All cell lines sequencing experiments were repeated in triplicate. Differential expression of each non-phosphorylatable CARM1 mutation expressing cell line was performed independently comparing to wild-type.