Deletion of Slc9a1 in Cx3cr1+ cells stimulated microglial subcluster CREB1-BDNF signaling and oligodendrocyte crosstalk

Microglial Na/H exchanger-1 (NHE1) protein, encoded by Slc9a1, plays a role in white matter demyelination of ischemic stroke brains. In this study, we conducted single cell RNA-seq transcriptome analysis of corpus collosum and external capsule from wild-type (WT) and conditional Slc9a1 knockout (cKO) mice at 3 days post-stroke to study microglia-oligodendrocyte interactions. Overall design: White matter tissues were dissected from the WT or Nhe1 cKO brains at 3 days post-tMCAO Grant ID: 933134 (Career Development Award) Grant title: Modulating microglia-oligodendrocyte interactions for white matter repair Funding source: American Heart Association Grantee: Shanshan Song