Intraspecific diversity in the mechanisms underlying abamectin resistance in a cosmopolitan pest

Villacis-Perez et al. (2023) uncovers the complex basis of abamectin resistance, and highlights the intraspecific diversity of genetic mechanisms underlying resistance in <em>Tetranychus urticae</em>. This study used experimental evolution in combination with a population-level based bulked segregant analysis (BSA) and with next-generation sequencing to map quantitative trait loci (QTL) associated with abamectin resistance in two genetically unrelated strains of <em>T. urticae, </em>ROS-ITi and MAR-ABi (Kurlovs et al., 2019). Results reveal how unrelated populations of a single arthropod species respond to abamectin selection, the (polygenic) structure of abamectin resistance, and the genes and mechanisms likely to underlie abamectin resistance. The Supplementary Data for Villacis-Perez et al. (2023) comprises: Data S1: genomic VCF (gzipped) used as input for the RUN_BSA1.02.py script available at https://github.com/rmclarklab/BSA (Kurlovs et al. 2019, PMID: 31499416); Data S2: RUN_BSA1.02.py commands using the gvcf in Data S1 as input.; Data S3: output of the RUN_BSA1.02.py script for aBSA (MAR-ABi); Data S4: output of the RUN_BSA1.02.py script for gBSA ROS-ITi.