RNA-seq of eight lymphoblastoid cell lines after AHR ligand treatments

RNA-seq was done on eight different lymphoblastoid cell lines for vehicle control, 3-MC agonist treatment and GNF-351 antagonist treatment for AHR to look at AHR regulated genes with different genetic backgrounds. We identified 69 genes that were highly differentially expressed after 3-MC or GNF-351 antagonist treatment with variation in the degree fo differential expression between the eight cell lines. Eight lymphoblastoid cells lines were treated in duplicate with vehicle control, 3-MC or GNF-351 for 24 hours before RNA collection. Libraries were prepared on the TruSeq RNA Library Prep Kit v2 and paired end sequencing was carried out on Illumina HiSeq 4000