Data from: The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs

Drug hypersensitivity reactions (DHR) are commonly observed with various
medications. However, some drugs, like Qingkailing (QKL), iodixanol, and
vancomycin, previously classified as pseudoallergenic, do not align with
clinical observations, creating uncertainty in understanding DHR
mechanisms and implementing prevention. In response, our research team
hypothesized that IgE-mediated allergy and pseudoallergic hypersensitivity
may synergistically lead to more severe reactions. We established an
IgE-mediated hypersensitivity model using an anti-dinitrophenyl IgE
monoclonal antibody in RBL-2H3 cells and employed ovalbumen (OVA) for
guinea pigs. Compound 48/80 served as a positive control for
non-immunologic hypersensitivity. We measured β-hexosaminidase and
histamine released in cells and guinea pigs to focus on hypersensitivity
responses from both IgE-mediated and pseudoallergic pathways. Results
indicated that combining pseudoallergenic drugs and IgE significantly
increased the hypersensitivity response compared to IgE or separate
compound 48/80 groups. This effect was mirrored in the OVA-induced model.
Notably, the three pseudoallergic drugs exhibited a similar increase in
IgE-mediated hypersensitivity. These findings highlight the additive
effects of IgE-mediated and pseudoallergic hypersensitivity, suggesting a
more potent DHR response. The study also measured several cytokines and
developed a method to differentiate between allergic, pseudoallergic DHRs,
and additive reactions, aiding in understanding and controlling serious
DHR risks in clinical practice.